-
Isolation and Culture of Primary Endothelial Cells
The purpose of this chapter is to describe the isolation techniques that result
-
Zymography and Reverse Zymography for Detecting MMPs and TIMPs
Zymography is the electrophoretic separation of proteins through a polyacrylamid
-
The Mitochondria of Cultured Mammalian Cells: II: Expression and Visualization of Exogenous Proteins
Mitochondria are almost ubiquitous organelles in Eukaryota. They are highly dyna
-
Quantitation of Amobarbital, Butalbital, Pentobarbital, Phenobarbital, and Secobarbital in Urine, Se
Barbiturates are central nervous system depressants with sedative and hypnotic p
-
Dopaminergic Neuronal Differentiation Protocol for Human Mesenchymal Stem Cells
The generation of dopamine (DA) neurons from stem cells holds great promise for
-
Testicular Germ Cell Tumors in Mice: New Ways to Study a Genetically Complex Trait
Testicular germ cell tumors (TGCTs) are the most common cancer affecting young m
-
In Vitro Derivation of Chondrogenic Cells from Human Embryonic Stem Cells
Human embryonic stem cells (hESCs) have the ability to self-renew and differenti
-
Cloning of Cytokine 3 Untranslated Regions and Posttranscriptional Assessment Using Cell-Based GFP A
Cytokine biosynthesis is tightly regulated by a number of processes, including g
-
Reporter/Functional Gene Transfer in Rat Brain
Of the many methods and techniques for in vivo gene transfer, some have already
-
Looking for the Niche: Substance Delivery into the Lateral Ventricle of the Brain: The Osmotic Minip
The infusion of different substances into the left ventricle of the brain allows
-
The Zebrafish: An Overview of Its Early Development
During the past 10 yr, the zebrafish has emerged as an important model system fo
-
The Use of Retroviral Vectors for tet-Regulated Gene Expression in Cell Populations
Today the treatment of inherited diseases holds a major field in gene therapy, a
-
Analysis of Ca2+-Binding Sites in the MthK RCK Domain by X-Ray Crystallography
Regulator of K+ conductance (RCK) domains form a conserved class of ligand-bindi
-
The Basic-Helix-Loop-Helix-Leucine Zipper Gene Mitf: Analysis of Alternative Promoter Choice and Spl
The activity of transcription factors is often regulated by Post-translational m
-
Histochemistry and Lipid Profiling Combine for Insights into Aging and Age-Related Maculopathy
Aging is the major risk factor for age-related maculopathy (ARM), the biggest ca
