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Identification and Assay of Allosteric Regulators of S-Adenosylmethionine Decarboxylase
Polyamine biosynthesis is extensively regulated in cells by multiple mechanisms,
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Investigation of Genomic Methylation Status Using Methylation-Specific and Bisulfite Sequencing Poly
Epigenetic modification plays a central role in the regulation of gene expressio
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Variations on the Single-Molecule Assay for Microtubule-Associated Proteins and Kinesins
The direct observation of single kinesins and microtubule-associated proteins (M
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单克隆抗体的制备与鉴定方法
相关专题 生物帮之抗体制备摘要:从人脐静脉分离的内皮细胞经培养后免疫 Balb/c小鼠,利用小鼠杂交瘤技术,建立了一株持续分泌抗人内皮细胞单克隆抗体的杂交瘤。对
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Derivation of Primary Mouse Embryonic Fibroblast (PMEF) Cultures
Primary mouse embryonic fibroblasts (PMEFs) have a number of properties that mak
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Glomerular Epithelial and Mesangial Cell Culture and Characterization
The advent of in vitro culture techniques has enabled the culture of homogeneous
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Matrix Assembly, Cell Polarization, and Cell Survival: Analysis of Peri-Implantation Development Wit
A variety of mutations, including those affecting laminin expression and basemen
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Isolation and Purification Method of Mouse Fetal Hepatoblasts
During development, liver precursors constitute a valuable source of pluripotent
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Genome-Wide Analysis of RNA Extracted from Isolated Mitochondria
Isolating mitochondria by subcellular fractionation is a well-established method
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Assays of Lipolytic Enzymes
Free fatty acids (FAs) derived from adipose tissue (AT) are, quantitatively, the
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Preparing Mitochondria from Rat Liver
Liver is a convenient source for functional intact mitochondria for a number of
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Isolation and Culture of Bone-Forming Cells (Osteoblasts) from Human Bone
The most conspicuous function of the osteoblast is the formation of bone. During
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Tracking Nanoparticles in Three-Dimensional Tissue-Engineered Models Using Confocal Laser Scanning M
Here we describe a method for imaging the position of nanoparticles within a 3D
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分子细胞生物学(molecular biology of the cell)
以细胞为对象, 主要在分子水平上研究细胞生命活动的分子机制, 即研究细胞器、生物大分子与生命活动之间的变化发展过程, 研究它们之间的相互关系, 以及它们与环境之
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细胞死亡(cell death)
细胞死亡的一般定义是细胞生命现象不可逆的停止。细胞死亡有两种形式:一种为坏死性死亡(necrosis),是由外部的化学、物理或生物因素的侵袭而造成的细胞崩溃裂解