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The Role of Redox in Signal Transduction
Functioning and efficient cell signaling is vital for the survival of cells. Ove
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Overview of Missing Data Techniques
Missing data frequently arise in the course of research studies. Understanding t
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Maintenance of Chicken Embryonic Stem Cells In Vitro
In this chapter, we describe the methods we have used to show that chicken leuke
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Dual-Channel Confocal Ratioing of Calcium Dynamics in Living Eggs and Oocytes
Changes in intracellular calcium concentrations ([Ca2+ ]i ) have been shown to r
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Human Tracheal Gland Cells in Primary Culture
For several years, tracheal gland cells have been cultured from different animal
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Pluripotent Stem Cells In Vitro from Human Primordial Germ Cells
In the mouse, embryonic germ (EG) cell lines can be generated from primordial ge
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Culture Systems: Air Quality
Poor laboratory air quality is a known hazard to the culture of human gametes an
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Overview: Structural Biology of Integrins
Integrins are cell adhesion molecules that play important roles in many biologic
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Renal Gene Therapy
Until now there is no renal gene therapy available for clinical use, however, ge
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Analyzing Association of the Endoplasmic Reticulum with the Legionella pneumophilaContaining Vacuole
A unique feature of the intracellular life cycle of Legionella pneumophila is th
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Enzyme Immobilization Using Chitosan-Xanthan Complexes
The immobilization of enzymes is a technique extensively studied since the late
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Isolation and Expansion of Mesenchymal Stem Cells/Multipotential Stromal Cells from Human Bone Marro
In recent years, human mesenchymal stem cells (multipotential stromal cells) fro
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Mammalian Membrane Receptors Expression as Inclusion Bodies in Escherichia coli
Integral membrane proteins, in particular receptors, regulate numerous physiolog
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Histone Deacetylase Activity Assay
Histone deacetylases (HDACs) are enzymes that catalyze the removal of acetyl gro
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Genetic Manipulation to Analyze Pheromone Responses: Knockouts of Multiple Receptor Genes
Gene targeting in the mouse is an essential technique to study gene function in
