-
组织FISH实验操作步骤
以癌组织为例，实验原理步骤如下：原位杂交的实验原理我们有提到过：用已标记的核酸探针与组织切片或细胞中的待测核酸中的互补序列杂交,从而对组织细胞中的核酸进行定性、
-
Methods for Data Analysis
The molecular epidemiology of infectious diseases uses a variety of techniques t
-
荧光原位杂交实验操作步骤
荧光原位杂交技（FISH）原理：是利用荧光标记的特异核酸探针与细胞内相应的靶DNA分子或RNA分子杂交，通过在荧光显微镜或共聚焦激光扫描仪下观察荧光信号，来确定
-
Micellar Electrokinetic Chromatography of Aminoglycosides
The components of the aminoglycosides, e.g., gentamicin, sisomicin, netilmicin,
-
Computational Modeling of Signal Transduction Networks: A Pedagogical Exposition
We give a pedagogical introduction to computational modeling of signal transduct
-
常用细胞凋亡检测方法（五）
七、凋亡相关蛋白TFAR19蛋白的表达和细胞定位分析 TFAR19（PDCD5）是由本研究室在国际上首先报导的一个拥有自己知识产权的人类新基因，前期的功能研
-
Engineering Human Mesenchymal Stem Cells to Release Adenosine Using miRNA Technology
Adenosine is an important modulator of metabolic activity with powerful tissue-
-
Identification and Quantitation of Cocaine, Benzoylecgonine, and Cocaethylene in Blood, Serum, and P
Cocaine is a widely abused stimulant. Numerous methods exist for the identificat
-
Rap1 and Integrin Inside-Out Signaling
In leukocytes, integrins play important roles in adhesive interactions with endo
-
Fluorometric Methods for Detection of Mitochondrial Membrane Permeabilization in Apoptosis
The mitochondrial regulation of cell death involves the release of proapoptotic
-
High-Resolution Scanning Electron Microscopy for the Imaging of Nuclear Pore Complexes and Ran-Media
High-resolution scanning electron microscopy provides three-dimensional surface
-
Aging of Cultured Human Skin Fibroblasts
There is substantial evidence that aging is related to the finite ability of som
-
原位杂交实验操作注意事项
1、组织取材：组织取材应尽可能新鲜。由于组织RNA降解较快，所以新鲜组织和培养细胞最好在30 min内固定。2、细胞组织固定目的与注意事项：（1）保持细胞结构；
-
Gametic Cell Adhesion and Fusion in the Unicellular Alga Chlamydomonas
Differentiation of vegetative cells of the haploid eukaryote Chlamydomonas is de
-
原位杂交实验操作常见问题答疑
Qusetion1：怎样保证FISH荧光原位杂交操作时的温度呢？答：有条件的情况下可以用荧光原位杂交的专用仪器进行操作。人工操作的话要注意：(1)对荧光原位杂交
