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研究新热点之细胞焦亡
细胞焦亡(Pyroptosis)又称细胞炎性坏死,是一种程序性细胞死亡,表现为细胞不断胀大直至细胞膜破裂,导致细胞内容物的释放进而激活强烈的炎症反应。细胞焦亡是
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Analysis of Janus Tyrosine Kinase Phosphorylation and Activation
Activation of Janus kinases (Jaks) occurs through autophosphorylation of key tyr
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细胞焦亡的研究方法与检测方式
首先是细胞焦亡的特点:细胞焦亡发生时,细胞会发生肿胀,在细胞破裂之前,细胞上形成凸出物(图1,早期细胞焦亡),之后细胞膜上形成孔隙,使细胞膜失去完整性,释放内容
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EMS Screens: From Mutagenesis to Screening and Mapping
The success of Drosophila as a genetic model organism is based on the efficient
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Generation and Analysis of Biosensors to Measure Mechanical Forces Within Cells
The inability to measure mechanical forces within cells has been limiting our un
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Confocal Microscopy of Live Xenopus Oocytes, Eggs, and Embryos
The use of the confocal microscope to study living Xenopus eggs affords the oppo
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细胞焦亡在临床各领域的研究现状
首先是细胞焦亡与感染性疾病,也是我们在对细胞焦亡的研究中最简单也最广泛的。 在病原体感染时,适度的细胞焦亡可清除致病微生物,而过度的细胞焦亡在导致细胞死亡
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In Vivo Detection of Protein-Protein Interaction in Plant Cells Using BRET
The emerging technique of bioluminescence resonance energy transfer (BRET) allow
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Quantitative Fluorescence In Situ Hybridization on Paraffin Embedded Tissue
Quantitative fluorescence in situ hybridization (Q-FISH) is a complex technique
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Fluorescence Immunohistochemistry in Combination with Differential Interference Contrast Microscopy
We have developed a technique, using a combination of immunofluorescent staining
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Exploring the Link Between Human Embryonic Stem Cell Organization and Fate Using Tension-Calibrated
Human embryonic stem cell (hESc) lines are likely the in vitro equivalent of the
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白色体(etioplast)
质体的一种,不含色素, 多存在于植物的分生组织和储藏组织中, 具有制造和储藏淀粉、蛋白质和油脂的功能。这类质体是由于前质体在发育分化过程中一直处于黑暗中,发育的
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原位杂交实验
原位杂交(in situhybridization)将标记的核酸探针与细胞或组织中的核酸进行杂交,称为原位杂交,使用DNA或者RNA探针来检测与其互补的另一条链
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Difficult Proteins
The degree of protein diversity and dynamic range within organisms means that ev
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Immunocytochemistry on Suprachiasmatic Nucleus Slices
Immunocytochemistry (ICC) is a sensitive and powerful method that is used to loc