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Isolation of Nucleic Acids
Thorough characterization of recombinant strains of Pichia pastoris requires ana
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Feeder-Free Substrates for Pluripotent Stem Cell Culture
A significant barrier to the therapeutic application of pluripotent stem cells (
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Recording hERG Potassium Currents and Assessing the Effects of Compounds Using the Whole-Cell Patch-
The complex gating of the hERG channel makes it ideally suited to its principal
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胞质分裂(cytokinesis)
有丝分裂后期, 将细胞膜、细胞骨架、细胞器,以及可溶性蛋白质等均等分配，并形成两个新的子细胞的过程称为胞质分裂。胞质分裂通常开始于有丝分裂后期，直到两个新细胞核
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Screening Expression Libraries with Solution-Based Assay
To date, the most common approach to screening an expression library for an RNA-
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Ras GTPases Are Both Regulators and Effectors of Redox Agents
Redox agents have been historically considered pathological agents which can rea
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Rearing Larvae of Sea Urchins and Sea Stars for Developmental Studies
Sea urchins have long been used to study morphogenesis and cell fate specificati
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停靠蛋白(docking protein, DP)
即SRP在内质网膜上的受体蛋白,它能够与结合有信号序列的SRP牢牢地结合,使正在合成蛋白质的核糖体停靠到内质网上来。停靠蛋白含有两个亚基，一个亚基暴露于细胞质的
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Laboratory Guidelines for Animal Care
Animal research is a controversial subject because of the ethical and moral impl
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Differentiate ES cells into cystic embryoid bodies
Differentiate ES cells into cystic embryoid bodiesDay -1: Pass ES cells at norma
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Induction of p53 Protein as a Marker for Ionizing Radiation Exposure In Vivo
The use of ionizing radiation as a therapeutic agent has been recognized for alm
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Apoptosis: Miniassay
1) Aliquot 5 X 106 total cells in 5-8 ml 2% media. 2) Add 30 µl 3H-thymidine. 3)
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Senescence Regulation by the p53 Protein Family
p53, a guardian of the genome, exerts its tumor suppression activity by regulati
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Molecular Profiling of the Epidermis: A Proteomics Approach
Mouse skin has been used extensively as a model system to study the development
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Application of Fluorescent Protein Tags as Reporters in Live-Cell Imaging Studies
The advent of the “GFP technology” together with advances in digital imaging and
