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Analysis of Gene-Specific and Genome-Wide Sperm DNA Methylation
Epigenetic modifications on the DNA sequence (DNA methylation) or on chromatin-a
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关于乳鼠心肌细胞培养的一点收获
在园子里浏览了很多次,发现关于乳鼠心肌细胞培养的资料还是几年前的比较多,不知道是不是这几年已经做得很少了。我是今年刚开始做的,发现虽然说方法已经很成熟了,但是做
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Quantitation of Tacrolimus in Whole Blood Using High Performance Liquid Chromatography-Tandem Mass S
We describe a multiple reaction monitoring positive ion HPLC/tandem mass spectro
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In Vivo Imaging of Oligonucleotidic Aptamers
In this chapter we present the methods developed in our laboratory for in vivo i
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Measurements of Peptide and Nonpeptide Secretory Products from Adipocytes
In addition to their metabolic function of storing triglycerides and releasing f
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Using FRET-Based Reporters to Visualize Subcellular Dynamics of Protein Kinase A Activity
The ubiquitous Protein Kinase A (PKA) signaling pathway is responsible for the r
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Neural Transplantation and Stem Cells
Recent results have raised important questions on our ability to amplify stem ce
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Determination of Gene Expression Patterns by In Situ Hybridization in Sections
In recent years, in situ RNA hybridization technique has found a widespread appl
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Gene Modification with Hapaxoterministic Restriction Enzymes: Easing the Way
Subcloning can be extraordinarily easy or extremely difficult. The simplest case
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Culture of Bone Marrow Mesenchymal Stem Cells on Engineered Matrix
Mesenchymal stem cells (MSCs) have huge potential to be used in cell therapies b
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Manipulating the Avian Epiblast and Epiblast-Derived Stem Cells
Compared to eutherian mammals, birds retain a primitive form of epiblast develop
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Isolating Aptamers Using Capillary ElectrophoresisSELEX (CESELEX)
SELEX (systematic evolution of ligands by exponential enrichment) is a process f
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Expansion of Pluripotent Stem Cells in Defined, Xeno-Free Culture System
The hallmark of pluripotent stem cells is their nearly unlimited self-renewal ca
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Cytofluorometric Purification of Diploid and Tetraploid Cancer Cells
During malignant transformation, cells can increase their ploidy and hence becom
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配子或终末减数分裂(Gametic or terminal meiosis)
此种减数分裂都是为了生成配子,减数分裂发生的时期是个体发育成熟之后。行此种减数分裂的生物包括所有的多细胞动物和多数原生生物。例如,在雄性脊椎动物中,在精母细胞分