组织学――组织制备

 Histological techniques (William H. Heidcamp) Very detailed guide to histological techniques, like  fixation, dehydration, embedment and subsequent sectioning.   

・         3-Aminopropyltriethoxysilane Treatment of Slides (Nottingham Pathology Lab)   

・         Paraffin-Embedded Tissue for LCM (Arcturus) Paraffin embedding is a process in which fixed (formalin or other fixative) is infiltrated and then blocked out in paraffin to stabilize it for long-term storage and easy sectioning...    

・         Wax embedding/sectioning (Klymkowsky Lab)   

• Chrome Gelatinised Slides and Coverslips (Nottingham Pathology Lab)    
• Decalcification (Nottingham Pathology Lab) Many surgical specimens contain calcified areas which need to be decalcified before processing and sectioning. This is the protocol used for this purpose   
• De-Paraffinization of Tissue Sections (Arcturus) Remove paraffin from section for tissue preparation such as nucleic acid extraction.    
• DNA Extraction From Paraffin Section (Arcturus) This extraction method has been used for measuring loss of heterozygosity (LOH), dideoxy fingerprinting (DDF), clonality analysis (chromosome X inactivation) and direct sequencing of PCR* products for single base mutational analysis. Extractions are typically performed on 500-1000 captured cells.   
• RNA Extraction from Paraffin Section (NIH Protocol) (Arcturus) A Protocol for extracting RNA from paraffin embedded tissue.   
• Preparation of Frozen Tissue for LCM (Arcturus)   
• White Blood Cell Cytospin (Arcturus)