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Microinjection into Xenopus Oocytes and Embryos
The ease of obtaining large numbers of Xenopus laevis eggs and oocytes together
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M-Current Recording from Acute DRG Slices
Electrophysiological recordings from an acutely sliced preparation provide infor
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前质体(proplastid)
前质体是质体的前身, 仅由内膜和外膜构成,膜内是少量的基质和DNA,但是没有LHC蛋白、叶绿素和电子传递系统。植物中的前质体随着在发育过程中所处的位置以及接受光
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The Problems and Pitfalls of Radioligand Binding
Radioligand binding is an extremely versatile technique that can be applied to a
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Differentiation of B Lymphocytes From Hematopoietic Stem Cells
Differentiation of B lymphocytes can be efficiently obtained when multipotent he
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贴壁细胞培养过程中支原体污染的几种救治方案
3.2 加温对支原体污染的治疗效果:经观察发现,加温8h后,荧光小点开始变少。16h细胞周围支原体几乎消失。进入24h开始细胞周围的荧光小点完全消失,但细胞开始
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High-Throughput Analyses of Gene Functions on a Cell Chip by Electroporation
Genome-wide functional annotation of genes is one of the major challenges in cur
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Disrupting Protein Complexes Using Tat-Tagged Peptide Mimics
Protein–protein interaction is a widely existing phenomenon and is essential for
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细胞培养目的与用途
1 科学研究:药物研究开发与基础研究 药物研究与开发 (1) 新药筛选:如化学合成药物药效研究,中药有效成分筛选与鉴定等. (2) 疫苗研究与开发:如病毒性疫苗
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细胞培养器械的清洗与消毒方法
相关专题 细胞培养相关用品 细胞培养专题【实验原理】在组织培养过程中，离体细胞对任何毒性物质都十分敏感。毒性物质包括解体的微生物 及细胞残余物以及非营养的化学物
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细胞生物基本方法：肌组织细胞培养
肌组织细胞培养1 ）骨骼肌细胞培养1． 杀死动物，无菌取大腿肌组织，切成 0.3 ～ 0.5 厘米小块。2． 用不含钙镁离子的 Hanks 液配的 0.25 ％
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Autophagy in Disease
Autophagy is a cellular quality control process by which cytoplasmic constituent
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Stem Cell Banks: Preserving Cell Lines, Maintaining Genetic Integrity, and Advancing Research
The ability to cryopreserve and successfully recover cell lines has been critica
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Mammalian In Vitro Splicing Assays
Splicing reactions are typically carried out using nuclear extracts, S100 extrac
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核剪接(nuclear splicing)
核剪接是指发生在细胞核中，主要是对hnRNA中内含子的剪接。这种剪接有三个主要特点∶一是在pre-mRNA中内含子与外显子间有特征性序列，即GT-AG规则；第二
