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Microspread Ovarian Cell Preparations for the Analysis of Meiotic Prophase Progression in Oocytes wi
Female fertility is critically influenced by two events affecting oocytes during
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In Vitro Generation of Human T Regulatory Cells: Generation, Culture, and Analysis of FOXP3-Transduc
T regulatory cells (Tregs) suppress immune responses and therefore have potentia
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Lentiviral Vector-Mediated Expression of pre-miRNAs and AntagomiRs
Micro (mi)RNAs are highly conserved small regulatory RNAs, which regulate gene e
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Determination of Phosphoinositide Binding to K+ Channel Subunits Using a ProteinLipid Overlay Assay
Phosphoinositides are an important component of the cell as they have a variety
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Volatile Oxylipins and Related Compounds Formed Under Stress in Plants
Plants form volatile oxylipins and related compounds under stress. Some of them
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Melanoblasts as Multipotent Cells in Murine Skin
Melanoblasts are melanocyte precursors that are derived from neural crest cells
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Developmental Biology Protocols: Overview II
The discipline of developmental biology covers scientific investigations aimed a
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微管组织中心(microtubule organizing centers, MTOC)
存在于细胞质中决定微管在生理状态或实验处理解聚后重新组装的结构叫微管组织中心。在多数情况下MTOC有一对中心粒和一个中心体, 但是某些表皮细胞和新受精的卵细胞，
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相差观察和摄影
相差观察和摄影 1） 相差装置的使用1． 先调好相位板，使聚光器相位板号与接物镜放大倍数相一致。2． 然后抽出接目镜，再换辅助望远镜，移动辅助镜筒并调整聚光器
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Use of Gene Overexpression to Assess Function in Cell Cycle Control
Transient overexpression of wild-type or mutant cell cycle regulatory factors is
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Using an Inverse PCR Strategy to Clone Large, Contiguous Genomic DNA Fragments
Conventional PCR screens of genomic DNA will often yield a substantial fragment
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Cell Cycle Analysis of Asynchronous Populations
Measurement of a DNA histogram can be achieved by fixing or permeabilizing cells
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Freeze-Fracture Electron Microscopy on Domains in Lipid Mono- and Bilayer on Nano-Resolution Scale
Freeze-fracture electron microscopy (FFEM) as a cryo-fixation, replica, and tran
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Methods for Determining the Proliferation of Cells in Response to EGFR Ligands
The evaluation of cell proliferation can be accomplished by several methods. The
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Splice Redirection as a Convenient Assay to Monitor CPPON Efficiency and Mechanism
Several strategies based on synthetic oligonucleotides (ON) have been proposed t
