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Coning Hybridoma cDNA by RACE
Hybridoma cDNA can be difficult to clone using V region PCR. This is due to muta
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Conjugation of Antibodies to Alkaline Phosphatase
Alkaline phosphatase is coupled to immunoglobin G antibody in a one-step procedu
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Conjugation of Antibodies to Biotin
Antibodies can be conjugated to biotin by a number of chemical means. They can t
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Conjugation of Antibodies to Fluorescein or Rhodamine
The isothiocyanate derivative of the fluorophore, fluorescein or rhodamine, is c
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Conjugation of Antibodies to Horseradish Peroxidase
Horseradish peroxidase is coupled to IgG antibody in a two-step procedure. In th
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Conjugation of Antibody to HRP
Reagents and Materials PBS PBS: 0.05M EDTA PBS: 0.01 thimerosal Conjugate Storag
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Conjugation of Colloidal Gold to Proteins
The ability to conjugate proteins to colloidal gold sols provides a wide variety
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Conjugation of Fluorochromes to Antibodies
The use of specific antibodies labeled with a fluorescent dye to localize substa
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Considerations in Generating Transgenic Mice: DNA, RNA, and Protein Extractions from TissuesRapid an
The mouse provides a powerful system to produce and study models of human diseas
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Construction and Production of an IgG-Like Tetravalent Bispecific Antibody for Enhanced Therapeutic
In past years, both laboratory and early clinical studies have demonstrated that
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Construction and Production of an IgG-Like Tetravalent Bispecific Antibody, IgGSingle-Chain Fv Fusio
In recent years, both laboratory and clinical studies have demonstrated that bis
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Construction and Screening of an Antigen-Derived Peptide Library Displayed on Yeast Cell Surface for
Identification of T cell epitopes is a critical, but often difficult step in stu
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Construction of a Human Antibody Domain (VH) Library
Highly diverse antibody (Fab or scFv) libraries have become vital sources to sel
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Construction of a Large Nave Human Phage-Displayed Fab Library Through One-Step Cloning
Antibody-based therapeutics is attracting more attention in the post-genome era,
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Construction of cDNA Libraries for the Analysis of the Structure of Complementary Strand Transcripts
The Epstein-Bar virus (EBV)-encoded complementary strand transcripts (CSTs) are
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